Offline Request Forms

To send your request, please follow the steps below

01

Download and fill in the Request Form and Patient Consent Form

To submit an Oncologica Test Request for your patient, please download the relevant form, complete all required fields, print and include it in the shipping package

 

02

Specimen and Packaging Requirements

All samples must be packaged in accordance with the Exempt Patient Specimens standards and labelled ‘NOT RESTRICTED AS PER IATA 3.6.2.2.3.8 EXEMPT HUMAN SPECIMEN’.

Remember: all samples must be accompanied by the histopathology report.

Paraffin block

One formalin-fixed paraffin –embedded (FFPE) block should be provided. The FFPE block is the routine pathological sample used by the pathologist to make the diagnosis and stored in the hospital pathology archive. The FFPE can be delivered to Oncologica through the routine postal delivery system or alternatively, we are happy to arrange a courier at no additional cost. This is the most preferable specimen as it will enable us to perform macro-dissection and/or the re-extraction of DNA/RNA if required.

Curls

If the block is not available, 10µm curls are acceptable. The number of curls required is dependent on the size of the block and/or tumour area. An unstained 3 µm section should also be sent for H&E staining so we can assess tumour percentage in the curl

  • For large specimens (>1.5cm2), 1x 10µm curl.
  • For medium-sized specimens (1-1.5cm2), 2 x 10µm curls.
  • For small specimens (0.5-1cm2), 3 x 10µm curls.
  • For specimens with multiple tissue fragments, 6-8 x 10µm curls..

All curls should be placed in one 1.5ml Eppendorf tube

Unstained slides

Unstained slides are acceptable. We require 10 x sections at 5 µm thickness, each placed on non-coated microscope slides and unbaked, 2 x unstained sections at 3 µm placed on coated microscope slides to perform the Immunofocus Test and 3 x unstained 3 µm section should also be sent for H&E staining so we can assess tumour percentage.

2.1

Specific requirements and recommendations

Please note the following recommendations

H&E REQUIREMENTS

If sending curls or unstained slides, please send a 3µm unstained sections for H&E (preferably cut at the same time as the curl or section) to enable us to assess the specimen for tumour area (to enable macro-dissection in the case of unstained slides) or areas of necrosis that may affect the interpretation of results.

If, on the rare occasion that it is not possible to include an H&E slide, it is essential that the Test Request Form includes an indication of the tumour area percentage.

03

Send the parcel to Oncologica

Send samples to the address indicated below:

Oncologica UK Ltd Suite 15-16, The Science Village, Chesterford Research Park, Cambridge, CB10 1XL.

 

Remember: all samples must be accompanied by the histopathology report.

 

COURIER SERVICE

We can arrange international shipment for you , call us +44 (0) 1223 785327 or contact us by mail at info@oncologica.com

04

Test workflow

Each request is assessed analyzed to verify compliance with requirements. After the assessment has been completed the Test begins in accordance with the following workflow.

IMMUNOHISTOCHEMISTRY OR IN SITU HYBRIDISATION REQUESTS

Please send either the paraffin block or sufficient unstained sections to perform the tests requested, including at least one spare slide in case repeat staining is necessary.

3-4µm sections must be placed on adhesive microscope slides and baked for no more than1 hour in a 60oC oven.